Exposure to acetaminophen and all its metabolites upon 10, 15, and 20 mg kg intravenous acetaminophen in very preterm infants Pediatric Research MOTRIN PM Pain Relief Caplets MOTRIN Initial Glutathione Depletion During Short Term Bed Rest: Pinpointing Synthesis and Degradation Checkpoints in the Glutamyl Cycle Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.