Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Glutamine vs. Glutathione: Understanding the Differences For Youth Understanding the Impact of Acetaminophen: Tylenol and Glutathione in Autism Functional imaging and neurochemistry identify in vivo neuroprotection mechanisms counteracting excitotoxicity and neurovascular changes in the hippocampus and visual cortex of obese and type 2 diabetic animal models Caramelo 2023