Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Silymarin prevents acetaminophen induced hepatotoxicity via up regulation of the glutathione conjugation capacity in mice ScienceDirect Serotonin Deficiency Exacerbates Acetaminophen Induced Liver Toxicity In Mice Scientific Reports How Glutathione is Depleted from the Body Suzy Cohen, RPh offers natural remedies to help you feel better now!